On this page we will add questions that we are often asked by researchers learning Bioinformatics
How can I upload large files to Galaxy?
Galaxy recommend uploading large files using FTP. Instructions and a video are provided on the following website. If uploading to the galaxy.eu server, make sure that you replace usegalaxy.org with usegalaxy.eu in the instructions below.
Installing an FTP client, such as FileZilla will help to transfer files.
How can I analyse data from GEO in R / RStudio?
There is a package called
GEOquery available through Bioconductor that will greatly help this process. It can be installed as follows.
We have created a tutorial to go through a workflow to analyse data from GEO
Alternatively, GEO provide a GEO2R tool that provide the code for you
How can I perform survival analysis?
The Winship Biostatistics and Bioinformatics Shared Resource (BBISR) of Emory University have developed a nice web interface for performing survival analysis
A much more comprehesive guide can be found here:-
Can I read data from Excel into R?
Yes, if you have
.xlsx file they can be read into R. The recommended approach would be to save then as
.csv files, and proceed as normal. Otherwise, the
readxl package can be used
## do this the first time if you don't have the package install.packages("readxl") library(readxl) data <- readxl("<YOUR_FILE_NAME_HERE>")
However, you may wish to consult this guide on data organisation to make sure your data are in a suitable form for analysis in R
Is there a package to do …. in R?
Aside from google, the main places to look would be Bioconductor (for Biological data):-
or the main R repository at CRAN
I get a
file not found error when trying to read a file into R
R is having problems with the file path or file name that you specified.
1) check the file name to make sure there are no typos
2) check that the file exists in your current working directory. The working directory can be printed to screen using
The recommended way to organise your files in RStudio is using R projects.
Any files that you want to analyse should be placed inside the project directory.
If you are still having problems, RStudio has an Import Dataset option through the file menu. This will read your file, and also print the R code that would be required.
How do I make a heatmap from my differentially-expressed genes
There is a heatmap tool available through Galaxy, and here is a tutorial
The Degust tool can also make heatmaps
In R, the
ComplexHeatmap packages are recommended for their flexibility. You will need to filter your count matrix to contain rows for just your genes of interest.
A recent Bitesize Bioinformatics video from Babraham Bioinformatics explains the process
Do you have any courses on ….?
Check our website for the courses that we currently run. All should have links to materials. We have now created a link to other resources online that you can check out